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. Author manuscript; available in PMC: 2021 Apr 28.
Published in final edited form as: Biochemistry. 2020 Apr 7;59(16):1565–1581. doi: 10.1021/acs.biochem.0c00098

Figure 3. FRET-detected Bending and Wrapping of Promoter DNA by RNAP as {CC} Ensemble Advances and Forms the Stable OC.

Figure 3.

Time course (log scale) of normalized FRET acceptor (Cy5) emission intensity after mixing Cy3-Cy5 dye-labelled λPR DNA (50 nM final) with E. coli RNAP (50 nM final) at 19°C and exciting FRET donor (Cy3) at 515nm. Both dye orientations are shown: Cy5(+14) Cy3(−100) ──, Cy3(+14) Cy5(−100) ──. The vertical dashed line at 10 s corresponds to the onset of OC formation at these conditions.