Skip to main content
. Author manuscript; available in PMC: 2021 Apr 28.
Published in final edited form as: Biochemistry. 2020 Apr 7;59(16):1565–1581. doi: 10.1021/acs.biochem.0c00098

Table 2.

FRET-Determined Distances between −100 and +14 Probe Positions on λPR Promoter DNA in CC Intermediates

Species FRET Acceptor
Relative Amplitudea 		FRET Efficiencyb Distance between
−100 and +14b,c
I1E 0.45 ± 0.27 0.14 ± 0.08 75 ± 9 Å
I1M 0.18 ± 0.16 0.059 ± 0.052 89 ± 14 Å
I1L 0.75 ± 0.30 0.24 ± 0.09 68 ± 6 Å
OC 1 0.32 b 63 Å c
a

Average of Cy5−100 and Cy5+14 results ± 1 SD (Table 1) expressed relative to OC.

b

FRET efficiencies and dye-dye distances for I1E, I1M and I1L are calculated from the OC values listed here, assuming the Forster distance Ro is the same for I1 intermediates as for OC.

c

OC distance is from ref. 33; uncertainty is ± 12%, which does not affect relative distances but does increase the uncertainty in the absolute distances for the I1 intermediates.