Figure 6.
NCC expression is not increased in the double-KO mice. (A) An immunoblot showing the expression of pNCC, tNCC, Kir4.1, and Nedd4-2 in WT (Kcnj10flox/flox, Nedd4lflox/flox, and Kcnj10flox/flox/Nedd4lflox/flox), Ks-Kir4.1 KO, Ks-Nedd4-2 KO, and Ks-Nedd4-2/Kir4.1 KO mice. Gapdh, glyceraldehyde-3-phosphate dehydrogenase; IB, immunoblot. A scatter graph summarizes the normalized band intensity of the (B) pNCC and (C) tNCC in WT (Kcnj10flox/flox, Nedd4lflox/flox, and Kcnj10flox/flox/Nedd4lflox/flox), Ks-Kir4.1 KO, Ks-Nedd4-2 KO, and Ks-Nedd4-2/Kir4.1 KO mice, respectively. The mean value and SEM are shown on the left of each row. Both male and female mice were used for the western blot, and the data obtained from male/female mice were normalized with corresponding male/female WT mice. *Significant difference (P=0.05) determined by t test; #value is significantly different between Ks-Kir4.1 KO and Ks-Nedd4-2/Kir4.1 KO mice.