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NCC expression is suppressed in Ks-Nedd4-2/Kir4.1 KO mice. Images show tNCC immunostaining in (A–C) WT (Nedd4lflox/flox/Kcnj10flox/flox), (D–F) Ks-Nedd4-2/Kir4.1 KO, (G–I) Ks-Nedd4-2 KO, and (J–L) Ks-Kir4.1 KO mice. The images in red squares in (A, D, G, and J) are magnified with intermediate (B, E, H, and K) and large resolution (C, F, I, and L). Original magnification, ×4 in A, D, G, and J; ×20 in B, E, H, and K; ×60 in C, F, I, and L.
