Figure 2.
The microbial peptide B7 induced anti-GBM GN in WKY rats. WKY rats were immunized with B7 (n=11), α3127–148 (n=12), or CFA (control, n=12) on day 0, and experiments ended on day 49. Disease was assessed by proteinuria (A), serum creatinine (B), serum urea (C), crescent formation (D), and infiltrating cells (E–G). Illustrative photomicrographs of glomeruli showing crescent formation by periodic acid–Schiff stain, immunofluorescent staining of linear IgG deposits, and cellular infiltrates of CD4+ T cells, CD8+ T cells, and macrophages (H). Infiltrating cells are expressed as cells per gcs for T cells and IOD per gcs for macrophages. No pulmonary lesion was observed (H). *P<0.05, **P<0.01, and *** P<0.001.