Figure 4.
HLA-DR15 (Dr15+) transgenic mice immunized with B7 developed anti-GBM GN. DR15+.Fcgr2b−/− mice were immunized with OVA323–339 (n=8), α3135–145 (n=8), or B7 (n=7) on days 0, 7, and 14, and experiments ended on day 42. Disease was assessed by albuminuria (A), BUN (B), glomerular IgG deposition (C), segmental glomerular necrosis (D), crescent formation (E), glomerular fibrin (F), and infiltrating cells (G–I). Illustrative photomicrographs of glomeruli showing necrosis and early crescent formation by periodic acid–Schiff stain, immunofluorescent staining of linear IgG deposits, and immunoperoxidase staining of fibrin deposition and cellular infiltrates with macrophages as an example (J). IgG deposition is expressed semiquantitatively on the basis of intensity; infiltrating cells are expressed as cells per gcs. *P<0.05, **P <0.01, and *** P<0.001.