Sequence alignment of B7, mouse α3135–145, and human α3127–148. Two of the four critical amino acids (V137, W140, G142, and F143) of mouse α3135–145 were identical to those of B7. Six of the seven critical residues of α3127–148 (W136, I137, L139, W140, G142, F143, and F145) were identical to those of B7.