Figure 4.

Regulation of UTA1 and UTA2 in Pax2/8 mutants. Adult kidney sections from control mice or mice carrying floxed alleles of either Pax2, Pax8, or Pax2 and Pax8 after tamoxifen treatment were immunostained with antibodies against Slc14a2 (red) or the brush border protein villin (green). Low-power images on the left show staining in the outer medulla (UTA2) of controls and single mutants, but not in the Pax2/8 double KOs. Villin marks the proximal tubule rich cortex. Higher-powered images through the inner medulla are shown on the right, with little detectable apical UTA1 in the Pax2/8 double KOs. Representative sections from three independently derived kidneys are shown for each genotype.