TABLE 3.
Detection and differentiation of RNA species of influenza A virus in nasal swab specimens collected from two live poultry market workers in Bangladesha
| Market | Date of specimen collection | Clinical manifestation | RNP CTb | infA CTb | vRNA CTc | +RNA CTc | Inf B CTb,d | NTCe | A(H9)b | Virus isolation |
|---|---|---|---|---|---|---|---|---|---|---|
| Hatirpul bazaar | 2/8/2017 | Asymptomatic | 29.78 | 35.72 | 25.99 | – | – | – | – | – |
| Kirpur kacha bazaar | 4/17/2018 | Asymptomatic | 27.31 | 36.13 | 28.26 | – | – | – | 37.4 | – |
a
–, undetectable.
b
TaqMan qPCR assay designed against the matrix gene of influenza A virus (infA), human RNase P housekeeping gene (RNP), or the hemagglutinin gene of the influenza A(H9). Both the infA and RNP assays were FDA-approved.
c
Fifteen cycles of PCR preamplification were performed to enrich on-bead targets (modification of step 5 shown in Fig. 1).
d
High concentrations of influenza B RNA (infB, CT = 12.6) were processed in parallel as a near-neighbor, nontarget control.
e
The same portion of nuclease-free water was used as a nontemplate control (NTC).