TABLE 1.
Validation of taxonomic classification of ATCC reference strainsa
| ATCC strain reference no. | Taxonomy |
LORCAN top consensus sequence |
SANGER consensus sequence leBIBI QBPP taxonomyb | LORCAN vs Sanger consensus sequence identity (%) | |
|---|---|---|---|---|---|
| LORCAN taxonomy | leBIBI QBPP taxonomyb | ||||
| 33560 | Campylobacter jejuni subsp. jejuni | Campylobacter jejuni | [Campylobacter lari subsp. concheus, Campylobacter jejuni subsp. jejuni*, Campylobacter jejuni subsp. doylei] (and 2 others) | [Campylobacter lari subsp. concheus, Campylobacter jejuni subsp. jejuni*, Campylobacter jejuni subsp. doylei] (and 2 others) | 99.77 |
| 43504 | Helicobacter pylori | Helicobacter pylori | [Helicobacter pylori*] | [Helicobacter pylori*] | 99.54 |
| 29212 | Enterococcus faecalis | Enterococcus faecalis | [Enterococcus faecalis*] | [Enterococcus faecalis*] | 100.00 |
| 25922 | Escherichia coli | Escherichia coli | [Escherichia marmotae, Escherichia fergusonii] Shigella flexneri* | [Shigella flexneri] | 99.57 |
| 49247 | Haemophilus influenzae | Haemophilus influenzae | [Haemophilus influenzae*] | [Haemophilus influenzae*] | 98.94 |
| 49226 | Neisseria gonorrhoeae | Neisseria gonorrhoeae | [Neisseria gonorrhoeae*] | [Neisseria gonorrhoeae*] | 100.00 |
| 27853 | Pseudomonas aeruginosa | Pseudomonas aeruginosa | [Pseudomonas tropicalis*, Pseudomonas aeruginosa, Pseudomonas hussainii] | [Pseudomonas tropicalis*, Pseudomonas indica, Pseudomonas aeruginosa] | 99.78 |
| 25923 | Staphylococcus aureus | Staphylococcus aureus | [Staphylococcus aureus subsp. anaerobius*] | [Staphylococcus argenteus, Staphylococcus aureus subsp. aureus, Staphylococcus schweitzeri*] (and 2 others) | 99.79 |
| 49619 | Streptococcus pneumoniae | Streptococcus pneumoniae | [Streptococcus pneumoniae*, Streptococcus pseudopneumoniae] | [Streptococcus mitis, Streptococcus pneumoniae*] | 99.79 |
| 29741 | Bacteroides thetaiotaomicron | Bacteroides thetaiotaomicron | [Bacteroides thetaiotaomicron*] | [Bacteroides thetaiotaomicron*] | 99.78 |
| 43055 | Eggerthella lenta | Eggerthella lenta | [Eggerthella lenta*] | [Eggerthella lenta*, Eggerthella timonensis] | 99.32 |
| 51299 | Enterococcus faecalis | Enterococcus faecalis | [Enterococcus faecalis*] | [Enterococcus faecalis*] | 100.00 |
| 8176 | Moraxella catarrhalis | Moraxella catarrhalis | [Moraxella canis, Moraxella catarrhalis*, Moraxella nonliquefaciens] | [Moraxella canis, Moraxella catarrhalis*] | 100.00 |
| BAA-1705 | Klebsiella pneumoniae | Klebsiella pneumoniae | [Klebsiella variicola, Klebsiella quasivariicola*] | [Klebsiella pneumoniae subsp. rhinoscleromatis*, Klebsiella quasipneumoniae subsp. quasipneumoniae] | 98.93 |
| 13637 | Stenotrophomonas maltophilia | Stenotrophomonas maltophilia | [Stenotrophomonas maltophilia*] | [Stenotrophomonas maltophilia] | 100.00 |
a
Samples were analyzed in parallel by Sanger sequencing and with the LORCAN approach. The resulting consensus sequences were submitted to the online taxonomic identification platform leBIBI QBPP.
b
Square brackets indicate proximal clusters. Asterisks indicate closest sequences based on patristic distances.