FIG 2.

Expression profiles of differentially expressed, functionally clustered genes (DAVID). The expression profiles of the genes within the cluster bracketed in Fig. 1 were analyzed using DAVID. The 30 genes that were grouped by DAVID into statistically significant functional clusters are plotted as a heat map, as described in Materials and Methods. Plotted are row Z-score-standardized log₂-transformed means of replicates for all samples. (A) Expression profiles of WT, DUBmut, and EndoUmut infections for type I interferons and interferon-stimulated genes (ISGs) that were clustered by DAVID analysis. (B) Line graphs of normalized read count values for select genes. Plotted are the average normalized counts for each gene over all four time points. (C) Expression profile of WT and DUBmut infections for type I interferons. The normalized counts from each infection group at 12 h postinfection (h p.i.) were subjected to statistical testing using two-tailed Student’s t tests. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, p < 0.0001; ns, not significant. Data are presented as means ± standard deviation (SD).