TABLE 8.

Similarity analyses of HFrV-1 open reading frames by BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) and HMMSCAN (https://www.ebi.ac.uk/Tools/hmmer/)

Parameter	ORF1 (RdRp, L gene)	ORF2 (glycoprotein, G gene)	ORF3 (nucleoprotein, N gene)
Protein size (amino acids)	2,144	660	503
Signal peptide	No	1–17	No
Transmembrane domain	2125–2142	542–563	No
Closest match by protein BLAST (identity at amino acid level)	L protein of Guangdong red-banded snake chuvirus-like virus, AVM87272.1 (48.56)	Putative glycoprotein of Guangdong red-banded snake chuvirus-like virus, AVM87273.1 (43.06)	Putative nucleoprotein of Guangdong red-banded snake chuvirus-like virus, AVM87274.1 (41.97)
Domains identified by protein BLAST (domain, amino acid region, E value)	Mononegavirales RNA-dependent RNA polymerase (72–1002, 4.25E−60), Mononegavirales mRNA-capping region V (1038–1282, 2.03E−12), mRNA-capping enzyme, Paramyxovirus family (1615–1855, 8.69E−3)	None	None
Protein Information Resource Superfamily (PIRSF) matches (accession no.; amino acid region; E value)	RNA-directed RNA polymerase, Paramyxoviridae type (PIRSF000830; 21–584, 594–1066, 1703–1985; 1.7E−24)	None	None
Pfam matches (accession no; amino acid region; E value)	Mononeg_RNA_pol (PF00946.19; 55–1013, 1708–1734; 2.1E−73, 0.22)	None	Hypoxia-inducible factor 1 (PF11413.8; 154–167; 3.8E−06)
	Mononeg_mRNAcap (PF14318.6; 1038–1284; 4.1E−19)		Arginine repressor, DNA binding domain (PF01316.21; 39–65, 283–310; 0.79, 6.4E−05)