TABLE 3.
Antibodies used for flow cytometric analysis of immune cell subsetsa
| Flow panels | |||
|---|---|---|---|
| Antibody | Cloneb | Companyb | Catalog no.b |
| Monocyte/DC basic | |||
| Mouse anti-human CD3 FITC | SP34-2 | BD | 556611 |
| Mouse anti-human CD20 FITC | 2H7 | BD | 556632 |
| Mouse anti-human CD16 PE | 3G8 | BioLegend | 302008 |
| Mouse anti-human CD11c PE-Cy7 | 3.9 | BioLegend | 301608 |
| Mouse anti-human CD14 APC | M5E2 | BioLegend | 301808 |
| Mouse anti-human CD123 PerCP-Cy5.5 | 6H6 | BioLegend | 306016 |
| Mouse anti-human HLA-DR APC-Cy7 | L243 | BioLegend | 307618 |
| NK | |||
| Mouse anti-human CD3 FITC | – | – | – |
| Mouse anti-human CD20 FITC | – | – | – |
| Mouse anti-human CD14 FITC | M5E2 | BioLegend | 301804 |
| Mouse anti-human CD56 PE-Cy7 | B159 | BD | 557747 |
| Mouse anti-human granzyme B Alex647* | GB11 | BioLegend | 515405 |
| Mouse anti-human CD159a PE | Z199 | Beckman Coulter | IM3291U |
| Mouse anti-human CD8a APC-Cy7 | RPA-T8 | BioLegend | 301016 |
| Mouse anti-human CD16 PerCP-Cy5.5 | 3G8 | BioLegend | 302028 |
| DC Activation | |||
| Mouse anti-human CD3 FITC | – | – | – |
| Mouse anti-human CD20 FITC | – | – | – |
| Mouse anti-human CD14 FITC | M5E2 | BioLegend | 301804 |
| Mouse anti-human HLA-DR APC-Cy7 | – | – | – |
| Mouse anti-human CD11c PE-Cy7 | – | – | – |
| Mouse anti-human CD123 PerCpCy5.5 | – | – | – |
| Mouse anti-human CD1c APC | AD5-8E7 | Miltenyi | 130-090-903 |
| Mouse anti-human CD86 PE | IT2.2 | BioLegend | 305406 |
| T cells | |||
| Mouse anti-human CD4 PerCp-Cy5.5 | OKT4 | Tonbo | 65-0048 |
| Mouse anti-human CD8b PE | 2ST8.5H7 | BD | 641057 |
| Mouse anti-human CD28 PE-Cy7 | CD28.2 | Tonbo | 60-0289 |
| Mouse anti-human CD95 APC | DX2 | BD | 558814 |
| Mouse anti-human CCR7 APC-Cy7 | G043H7 | BioLegend | 353212 |
| Mouse anti-human Ki67 FITC* | B56 | BD | 556026 |
| B cells | |||
| Mouse anti-human CD20 PE-Cy7 | 2H7 | eBioscience | 25-0209-73 |
| Mouse anti-human CD27 APC | O323 | Tonbo | 20-0279 |
| Mouse anti-human IgD PE | IA6-2 | Biolegend | 348204 |
| Mouse anti-human CD40 APC-Cy7 | 5C3 | BioLegend | 334324 |
| Mouse anti-human Ki67 FITC* | – | – | – |
| Mouse anti-human IgG PerCP-Cy5.5 | HP6017 | BioLegend | 409312 |
a
*Denotes intracellular antibody.
b
–, Same as monocyte/DC basic panel.