TABLE 1.
Primers for generating ALV-J infectious clones through homologous recombination technique
| Target infectious clone | Template/primer name and sequence (position of primer) (fragment size) for amplifying: |
|
|---|---|---|
| Target gene fragments | Linearized vectors | |
| EAV-HP | pc-EAV-HP-env/J-gp37-IC-F: CGGCACGCGGTGTTGCAGAATCGAGCAGCCATCGATTTC (bp 220–243 of EAV-HP-gp37); EAV-gp37-IC-R: CGCGTTCGGAACCTACAGCTGCTCCCTAATTCTAT (bp 572–591 of EAV-HP-gp37) (402 bp) | J1/S2-IC-F: TAGGTTCCGAACGCGATGTAACGGGGCAAG (bp 7009–7038 of J1); S2-IC-R: CAACACCGCGTGCCGGATGCTGTTCATGTC (bp 6607–6636 of J1) (9,921 bp) |
| 4817 | pc-4817-env/J-gp37-IC-F: CGGCACGCGGTGTTGCAGAATCGAGCAGCCATCGATTTC (bp 220–243 of 4817-gp37); 4817-gp37-IC-R: CGCGTTCGGAACCTATACTGCCCCATTTTCGGGCTG (bp 220–243 of 4817-gp37) (438 bp) | J1/S2-IC-F: TAGGTTCCGAACGCGATGTAACGGGGCAAG (bp 7009–7038 of J1); S2-IC-R: CAACACCGCGTGCCGGATGCTGTTCATGTC (bp 6607–6636 of J1) (9,921 bp) |
| YF-J1 | J1/J-gp37-IC-F: CGGCACGCGGTGTTGCAGAATCGAGCAGCCATCGATTTC (bp 6622–660 bp of J1); YF-J1R: TCCCTAATTCTATGAaATCTTATGCGTTCATCCATGAACTG (bp 6961–7001 of J1) (380 bp) | J1/YF-S2-IC-F: TCATAGAATTAGGGAGCAACTGTAGGTTCC (bp 6987–7016 of J1); S2-IC-R: CAACACCGCGTGCCGGATGCTGTTCATGTC (bp 6607–6636 of J1) (9,943 bp) |
| YN-J1 | J1/J-gp37-IC-F: CGGCACGCGGTGTTGCAGAATCGAGCAGCCATCGATTTC (bp 6622–6660 of J1); YN-J1R: CATCCATGAACTGATtCATTGTTCTTGATAGACAGTCCTGG (bp 6633–6973 of J1) (352 bp) | J1/YN-S2-IC-F: ATCAGTTCATGGATGAACGCATAAGATATC (bp 6959–6988 of J1); S2-IC-R: CAACACCGCGTGCCGGATGCTGTTCATGTC (bp 6607–6636 of J1) (9,971 bp) |
| NY-EAV-HP | EAV-HP/NY-EAV-HP-F: CTATTGAGAACAATGtATCAGTTTATGGATGAACGC (bp 6943–6978 of EAV-HP); NY-EAV-HP-R: GTACCACCTTACTTCCACCAATCAACGGGTACAGTGCC (bp 7341–7378 of EAV-HP) (436 bp) | EAV-HP/NY-EAV-HP-IC-F: GAAGTAAGGTGGTACGGTCATGGTATGATC (bp 7364–7393 of EAV-HP); NY-EAV-HP-IC-R: CATTGTTCTCAATAGACAATCCTGAAAGCAC (bp 6927–6957 of EAV-HP) (9,887 bp) |
| YF-EAV-HP | EAV-HP/J-gp37-IC-F: CGGCACGCGGTGTTGCAGAATCGAGCAGCCATCGATTTC (bp 6622–6660 of EAV-HP); YF-EAV-HP-R: TCCCTAATTCTATGAaATTTTATGCGTTCATCCATAAACTG (bp 6967–7001 of EAV-HP) 380 bp | EAV-HP/YF-EAV-HP-IC-F: TCATAGAATTAGGGAGCAGCTGTAGGTTC (bp 6987–7015 of EAV-HP); S2-IC-R: CAACACCGCGTGCCGGATGCTGTTCATGTC (bp 6607–6636 of EAV-HP) (9,943 bp) |
| YN-4817 | 4817/J-gp37-IC-F: CGGCACGCGGTGTTGCAGAATCGAGCAGCCATCGATTTC (bp 6622–6660 of 4817); YN-4817-R: CATCCATAAACTGATtCATTGTTCTCGATAGACAATCCTGG (bp 6933–6957 of 4817) (336 bp) | 4817/YN-4817-IC-F: ATCAGTTTATGGATGAACGCATCAGCTATCAC (bp 6959–6990 of 4817); S2-IC-R: CAACACCGCGTGCCGGATGCTGTTCATGTC (bp 6607–6636 bp of 4817) (10,007 bp) |
| 2YF-4817 | 4817/J-gp37-IC-F: CGGCACGCGGTGTTGCAGAATCGAGCAGCCATCGATTTC (bp 6622–6660 of 4817); 2YF-4817-R: ACAAGCCTTTTGCAACTTCTTGaATTCCGCGTGAaAGCT (bp 6982–7020 of 4817) | 4817/2YF-4817-IC-F: TTGCAAAAGGCTTGTAGGCAGCCCGAAAATG (bp 7006–7036 of 4817); S2-IC-R: CAACACCGCGTGCCGGATGCTGTTCATGTC (bp 6607–6636 of 4817) (9,960 bp) |
| NFF-4817 | 2YF-4817/J-gp37-IC-F: CGGCACGCGGTGTTGCAGAATCGAGCAGCCATCGATTTC (bp 6622–6660 of 2YF-4817); YN-4817-R: CATCCATAAACTGATTCATTGTTCTCGATAGACAATCCTGG (bp 6933–6957 of 2YF-4817) (336 bp) | 2YF-4817/NFF-4817-IC-F: ATCAGTTTATGGATGAACGCATCAGCTtTCAC (bp 6959–6990 of 2YF-4817); S2-IC-R: CAACACCGCGTGCCGGATGCTGTTCATGTC (bp 6607–6636 of 2YF-4817) (10,007 bp) |