TABLE 2.
Homologous genesa shared between Dishui Lake virophages and large/giant viruses
| Virophage ORF (no. of amino acids) | Homology with giant protozoa virus (NCBI protein accession no.) (alignment length/identity/E value) | Homology with large/giant algae virus (NCBI protein accession no.) (alignment length/identity/E value) | Homology with DSLPVs/DSLLAV1-ORF no. (alignment length/identity/E value) |
|---|---|---|---|
| DSLV1 | |||
| ORF01 (261) | Pithovirus LCPAC001 hypothetical protein (QBK89714.1) (203/28%/3e-14) | — | — |
| ORF02 (288) | — | Bathycoccus sp. RCC1105 virus hypothetical protein-( ADQ91335.1) (202/27%/5e-18) | DSLPV4-ORF64 (181/30%/2e-20) |
| ORF25 (173) | — | Paramecium bursaria Chlorella virus NY2A hypothetical protein ( YP_001497873.1) (118/45%/5e-36) | — |
| DSLV2 | |||
| ORF01 (260) | Pithovirus LCPAC001 hypothetical protein (QBK89714.1) (198/32%/1e-17) | — | — |
| ORF06 (196) | — | Chrysochromulina ericina virus hypothetical protein ( YP_009173402.1) (173/34%/2e-22) | — |
| ORF09 (218) | — | - | — |
| ORF10 (306) | — | Heterosigma akashiwo virus 01 hypothetical protein (AOM63366.1) (210/28%/1e-15) | — |
| ORF37 (107) | Marseillevirus Shanghai 1 hypothetical protein MarSH_451 ( AVR53156.1) (82/41%/4e-13) | — | — |
| ORF38 (343) | Marseillevirus LCMAC202 nucleotidyl transferase ( QBK88130.1) (342/26%/5e-13) | — | DSLLAV1-ORF214 (338/21%/5e-8) |
| DSLV3 | |||
| ORF01 (264) | Pithovirus LCPAC001 hypothetical protein (QBK89714.1) (224/30%/1e-19) | — | — |
| ORF17 (328) | — | — | DSLLAV1-ORF315 (225/23%/3e-6) |
| ORF22 (220) | Mimivirus AB-566-O17 hypothetical protein (ARR74930.1) (172/22%/3e-7) | Phaeocystis globosa virus hypothetical protein ( YP_008052689.1) (158/25%/1e-13) | DSLPV4-ORF64 (219/23%/3e-17) |
| ORF24 (271) | Pithovirus sibericum DNA-cytosine methyltransferase ( YP_009001166.1) (250/34%/1e-36) | Aureococcus anophagefferens virus cytosine-5 methyltransferase- ( YP_009052204.1) (248/32%/1e-34) | — |
| DSLV4 | |||
| ORF01 (268) | Pithovirus LCPAC001 hypothetical protein (QBK89714.1) (210/27%/2e-13) | — | — |
| ORF10 (130) | — | Paramecium bursaria Chlorella virus NY2A hypothetical protein (YP_001497873.1) (130/44%/5e-37) | — |
| ORF20 (131) | — | Heterosigma akashiwo virus 01 hypothetical protein ( AOM63366.1) (136/30%/2e-8) | DSLLAV1-ORF315 (117/27%/3e-5) |
| ORF26 (206) | — | Micromonas pusilla virus SP1 hypothetical protein ( AET84892.1) (189/26%/1e-16) | DSLPV4-ORF64 (196/26%/2e-20) |
| DSLV5 | |||
| ORF01 (253) | Pithovirus LCPAC001 hypothetical protein ( QBK89714.1) (196/31%/4e-18) | — | — |
| ORF02 (244) | Mimivirus AB-566-O17 hypothetical protein ( ARR74930.1) (170/26%/4e-14) | — | DSLPV4-ORF64 (182/26 %/1e-15) |
| ORF08 (312) | — | Heterosigma akashiwo virus 01 hypothetical protein (AOM63366.1) (189/25%/1e-10) | — |
| ORF10 (849) | — | Aureococcus anophagefferens virus putative D5-ATPase-helicase ( YP_009052397.1) (534/24%/1e-28) | — |
| DSLV6 | |||
| ORF01 (270) | Pithovirus LCPAC001 hypothetical protein (QBK89714.1) (218/25%/7e-12) | — | — |
| ORF15 (488) | — | Paramecium bursaria Chlorella virus glycoprotein repeat domain-containing protein ( AGE59082.1) (420/51%/3e-89) | DSLPV4-ORF180 (376/45%/1e-80) |
| ORF17 (324) | — | Heterosigma akashiwo virus 01 hypothetical protein (AOM63366.1) (218/26%/3e-14) | — |
| ORF24 (210) | — | Bathycoccus sp. RCC1105 virus hypothetical protein ( YP_004061590.1) (220/25%/2e-15) | DSLPV4-ORF64 (194/26%/2e-19) |
| DSLV7 | |||
| ORF01 (260) | Pithovirus LCPAC001 hypothetical protein (QBK89714.1) (203/28%/4e-14) | — | — |
| ORF05 (173) | — | Paramecium bursaria Chlorella virus NY2A hypothetical protein (YP_001497873.1) (118/45%/ 5e-36) | — |
| ORF30 (302) | — | Bathycoccus sp. RCC1105 virus hypothetical protein (ADQ91335.1) (202/27%/9e-18) | DSLPV3-ORF157 (201/27%/6e-12) |
| DSLV8 | |||
| ORF01 (253) | Pithovirus LCPAC001 hypothetical protein (QBK89714.1) (196/31%/4e-18) | — | — |
| ORF02 (244) | Mimivirus hypothetical protein (ARR74930.1) (170/26 %/4e-14) | Bathycoccus sp. RCC1105 virus hypothetical protein (ADQ91335.1) (211/25%/5e-14) | DSLPV4-ORF64 (182/26%/1e-15) |
| ORF08 (312) | — | Heterosigma akashiwo virus 01 hypothetical protein (AOM63366.1) (189/25%/1e-10) | — |
| ORF10 (849) | — | Aureococcus anophagefferens virus D5-ATPase-helicase (YP_009052397.1) (534/24%/1e-28) | — |
a
E value, <10−5; coverage, >60%. Nonredundant homologous genes are shown in boldface. –, Not determined.