FIG 6.

Characterization of long terminal repeats (LTRs) of KoRV in parents and joey. (A) Amplification of LTR gene using genomic DNA from the sire, dam, and joey. Lane NTC, no-template control; lane M, 1-kb marker (GeneDireX, Inc.). (B) A phylogenetic tree was constructed based on the nucleotide sequences of KoRV LTR using the MEGA7 software neighbor-joining method. The scale bar at the bottom indicates the nucleotide distance. (C) LTR multiple-sequence alignment. Black boxes indicate nucleotide differences between the joey with dam only, and the red box indicates nucleotide differences of baby from both the sire and dam. Asterisks indicate conserved sequences.