FIG 7.

DRB2 overexpressing PVX induces necrosis. (A) Infections of wild-type N. benthamiana with the indicated recombinant PVX viruses were initiated by agroinfiltration. Pictures of infected plants were taken at 14 dpi. (B) At 7 dpi protein lysates and total RNA were prepared from systemically infected leaves of N. benthamiana. Presence of PVX RNA was monitored by Northern blotting using radioactively labeled PVX-CP probe. The ethidium bromide (EtBr)-stained gel is shown as a loading control. Viral CP and DRB protein expression levels were analyzed by Western blotting using the indicated antibodies. The Ponceau-stained filter is shown as a loading control. PVX vsiRNA levels were analyzed by small-RNA Northern blotting. The filter was probed with radioactively labeled PVX-CP probe. The ethidium bromide-stained gel is shown as a loading control.