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The effects of BGLF2 and its herpesvirus orthologs on p-STAT3 and p-p38. 293T cells were transfected with plasmids expressing EBV BGLF2, HSV-1 UL16, VZV ORF44, or CMV UL94 tagged with V5-tag at their C terminus or empty vector pcDNA3.1 (vector control). After 48 h, the cells were treated with IFN-β (1,000 U/ml) for 20 min, and cell lysates were immunoblotted with antibody to p-STAT3, STAT3, p-p38, V5, and actin.
