TABLE 2.
MICs of cefepime, ceftolozane, and meropenem alone and in combination with BLIs against the panel of engineered P. aeruginosa strains producing various cloned beta-lactamasesa
| Strain | Beta-lactamase | MIC (μg/ml) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cefepime |
Ceftolozane |
Meropenem |
||||||||
| Alone | With BLIs |
Alone | With BLIs |
Alone | With BLIs |
|||||
| AVI | QPX7728 | AVI | QPX7728 | AVI | QPX7728 | |||||
| PAM4175 | vector | 0.125 | ≤0.06 | ≤0.06 | 0.25 | 0.25 | 0.125 | ≤0.06 | ≤0.06 | ≤0.06 |
| PAM4819 | CTX-M-2 | >64 | 0.25 | ≤0.06 | 4 | 0.125 | ≤0.06 | 0.125 | 0.125 | ≤0.06 |
| PAM4743 | CTX-M-15 | >64 | 0.25 | ≤0.06 | 64 | 0.25 | 0.125 | 0.5 | ≤0.06 | ≤0.06 |
| PAM4820 | CTX-M-25 | 2 | 0.125 | ≤0.06 | 16 | 0.25 | 0.125 | 0.5 | ≤0.06 | ≤0.06 |
| PAM4822 | CTX-M-27 | 32 | 0.125 | ≤0.06 | 64 | 0.25 | 0.25 | 0.5 | ≤0.06 | ≤0.06 |
| PAM4886 | GES-1 | 1 | 0.125 | ≤0.06 | 32 | 0.5 | 0.125 | 0.25 | ≤0.06 | 0.125 |
| PAM4800 | GES-19 | 0.5 | ≤0.06 | ≤0.06 | >64 | 0.5 | 0.125 | 1 | ≤0.06 | 0.125 |
| PAM4840 | OXY-6-2 | >32 | 0.25 | ≤0.06 | 1 | 0.25 | 0.125 | 0.25 | ≤0.06 | ≤0.06 |
| PAM4842 | PER-2 | >64 | 0.25 | ≤0.06 | >64 | 1 | 0.125 | 0.125 | ≤0.06 | ≤0.06 |
| PAM4907 | PER-4 | 32 | ≤0.06 | ≤0.06 | 32 | 32 | ≤0.06 | 0.125 | 0.125 | ≤0.06 |
| PAM4874 | SHV-12 | >64 | 0.125 | ≤0.06 | 16 | 0.25 | 0.125 | 0.25 | ≤0.06 | ≤0.06 |
| PAM4878 | TEM-10 | >64 | 0.125 | ≤0.06 | 16 | 0.25 | 0.125 | 0.25 | ≤0.06 | ≤0.06 |
| PAM4908 | VEB-1 | >64 | 0.125 | ≤0.06 | >64 | 0.5 | ≤0.06 | 0.25 | ≤0.06 | ≤0.06 |
| PAM4910 | VEB-2 | 16 | 0.125 | ≤0.06 | >64 | 0.5 | ≤0.06 | 0.25 | ≤0.06 | ≤0.06 |
| PAM4912 | VEB-3 | 64 | 0.25 | ≤0.06 | >64 | 0.5 | ≤0.06 | 0.5 | ≤0.06 | ≤0.06 |
| PAM4938 | VEB-9 | 0.5 | 0.125 | ≤0.06 | 256 | 2 | ≤0.25 | 0.13 | ≤0.06 | ≤0.06 |
| PAM4135 | KPC-2 | 4 | 0.125 | ≤0.06 | 8 | 0.25 | 0.125 | 64 | ≤0.06 | ≤0.06 |
| PAM4689 | KPC-3 | 32 | 0.25 | ≤0.06 | 32 | 0.5 | 0.125 | 64 | ≤0.06 | ≤0.06 |
| PAM4794 | NMC-1 | 64 | 0.125 | ≤0.06 | 0.5 | 0.25 | 0.125 | 64 | 0.125 | ≤0.06 |
| PAM4864 | SFC-1 | 16 | ≤0.06 | ≤0.06 | 2 | 0.25 | 0.125 | 64 | 0.125 | ≤0.06 |
| PAM4801 | GES-20 | >64 | 8 | ≤0.06 | 4 | 0.5 | 0.25 | 16 | ≤0.06 | ≤0.06 |
| PAM4744 | SME-2 | >64 | 0.25 | ≤0.06 | 0.5 | 0.25 | 0.125 | 16 | ≤0.06 | ≤0.06 |
| PAM4938 | VCC-1 | >64 | 0.25 | ≤0.06 | ND | ND | ND | 8 | ≤0.06 | ≤0.06 |
| PAM4676 | BKC-1 | 128 | 2 | ≤0.25 | 2 | 0.25 | 0.125 | 4 | ≤0.06 | ≤0.06 |
| PAM4186 | CMY-2 | 1 | ≤0.06 | ≤0.06 | 2 | 0.25 | 0.125 | 0.25 | ≤0.06 | ≤0.06 |
| PAM4825 | MIR-1 | 2 | 0.125 | ≤0.06 | 4 | 0.25 | 0.125 | 0.5 | ≤0.06 | ≤0.06 |
| PAM4745 | P99 (ECL chAmpC) | 8 | 0.125 | ≤0.06 | 8 | 0.25 | 0.125 | 0.5 | ≤0.06 | ≤0.06 |
| PAM4869 | PDC-1 (Pa chAmpC) | 4 | 0.125 | ≤0.06 | 1 | 0.25 | 0.125 | 0.5 | ≤0.06 | ≤0.06 |
| PAM4884 | ADC-181 (AB chAmpC) | 0.5 | ≤0.06 | ≤0.06 | 2 | 0.25 | 0.125 | 0.125 | ≤0.06 | ≤0.06 |
| PAM4827 | OXA-1 | 1 | ≤0.06 | ≤0.06 | 0.125 | 0.125 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 |
| PAM4792 | OXA-2 | 0.5 | 0.125 | ≤0.06 | 8 | 0.25 | 0.125 | 2 | 0.5 | ≤0.06 |
| PAM4846 | OXA-9 | 1 | 0.125 | ≤0.06 | 0.5 | 0.25 | 0.125 | 0.25 | ≤0.06 | ≤0.06 |
| PAM4790 | OXA-10 | 0.125 | ≤0.06 | ≤0.06 | 0.25 | 0.25 | 0.125 | 0.125 | ≤0.06 | ≤0.06 |
| PAM4217 | OXA-48 | 0.25 | ≤0.06 | ≤0.06 | 0.25 | 0.25 | 0.125 | 4 | ≤0.06 | ≤0.06 |
| PAM4875 | OXA-23 | 2 | 0.25 | ≤0.06 | 0.25 | 0.25 | 0.125 | 2 | 0.5 | ≤0.06 |
| PAM4876 | OXA-72 | 2 | 0.25 | ≤0.06 | 0.25 | 0.25 | 0.125 | 2 | 1 | ≤0.06 |
| PAM4877 | OXA-58 | 0.125 | ≤0.06 | ≤0.06 | 0.25 | 0.25 | 0.125 | 0.25 | ≤0.06 | ≤0.06 |
| PAM4179 | NDM-1 | >64 | >64 | 2 | >64 | >64 | 32 | 32 | 32 | 1 |
| PAM4917 | NDM-7 | ND | ND | ND | >64 | >64 | 32 | 32 | 32 | 1 |
| PAM4795 | VIM-1 | 64 | 64 | 0.25 | >64 | >64 | 1 | 8 | 4 | ≤0.06 |
| PAM4798 | VIM-2 | 8 | 4 | ≤0.06 | >64 | >64 | 0.25 | 8 | 8 | ≤0.06 |
| PAM4881 | VIM-7 | 0.25 | 0.125 | ≤0.06 | 4 | 2 | 0.125 | 1 | 1 | ≤0.06 |
| PAM4887 | IMP-1 | 64 | 64 | 1 | >64 | >64 | 2 | 8 | 4 | 0.25 |
| PAM4888 | IMP-4 | 32 | 32 | 1 | >64 | 64 | 2 | 4 | 4 | 0.25 |
| PAM4196 | IMP-13 | 16 | 16 | 1 | 64 | 32 | 2 | 1 | 1 | 0.5 |
| PAM4198 | IMP-15 | 16 | 16 | 0.5 | 64 | 64 | 1 | 1 | 1 | 0.125 |
| PAM4890 | IMP-19 | >64 | 32 | 2 | >64 | >64 | 4 | 1 | 1 | 4 |
| PAM4889 | IMP-26 | 64 | 64 | 8 | >64 | >64 | 16 | 8 | 8 | 2 |
| PAM4879 | CcrA | 16 | 16 | ≤0.06 | 4 | 4 | 0.125 | 2 | 2 | ≤0.06 |
| PAM4883 | GIM-1 | 8 | 8 | 0.25 | 32 | 32 | 0.5 | 32 | 16 | 0.5 |
| PAM4885 | SPM-1 | >64 | >64 | 64 | >64 | >64 | >64 | 64 | 64 | 32 |
| PAM4880 | L1 | 4 | 2 | 1 | 64 | 64 | 16 | 64 | 64 | 32 |
a
1A-CRB, class A carbapenemase; 2D-CRB, class D carbapenemase; BLIs, beta-lactamase inhibitors; AVI, avibactam; ND, not determined. All BLIs were tested at a fixed concentration of 4 μg/ml. Boldface indicates MIC to antibiotic alone.