TABLE 4.
Colistin MIC and expression levels of pmrB and pmrK genes of different E. coli strains and transformants carrying pCRII-TOPOpmrBΔ6-11
| Strain | Chromosomal pmrB status | pCRII-TOPOpmrB status | Colistin MIC (mg/liter) | Relative expression levela |
|
|---|---|---|---|---|---|
| pmrB | pmrK | ||||
| MG1655 | WT | None | 0.25 | 1 | 1 |
| EC3000 | Δ6-11 (RPISLR) | None | 8 | 15.48 ± 9.36 | 127.47 ± 35.14 |
| EC3000_ΔpmrBΔ6-11 | Deleted | pmrBΔ6-11 (RPISLR) | 0.25 | <0.01 | 3.25 ± 1.08 |
| EC3000_ΔpmrBΔ6-11 (pCRII-TOPOpmrBΔ6-11) | Deleted | None | 8 | 1.76 ± 0.14 | 10.18 ± 2.14 |
| EC3000 revertant | WT | None | 0.25 | 0.89 ± 0.43 | 2.21 ± 0.25 |
a
Expression levels (fold change) were normalized against the value obtained with MG1655. Values are means ± SD.