Fig 1.
Deletion of stp1 facilitates resistance in SF8300ex. (A) CFU/ml of knockout strains (Δstk1 and Δstp1) and wild-type SF8300ex after 4 h of treatment with 0.25 μg/ml nafcillin. (B) Ratio of survival of Δstp1-complemented strains after 4 h of treatment with 0.25 μg/ml nafcillin. Δstp1 complemented with empty-vector pTXΔ (E), wild-type stp1 (wt), mutant G169S stp1 (SRB), and mutant Q31X stp1 (SRT). *, P < 0.01; **, P < 0.009; ***, P < 0.0008; ****, P < 0.0001. Student's t test was used to analyze the P values using GraphPad Prism software. All experiments were repeated at least twice. ns, not significant.