Fig 2.
Loss of function of Stp1 reduces hemolytic activity of SF8300ex. (A) G169S mutation annotated in the crystal structure of Stp1 bound to metals (purple spheres). The figure was generated using UCSF Chimera and PDB entry 5F1M. (B) Decrease in hemolysis in Δstp1 strains expressing mutant Stp1s. Δstp1 complemented with empty-vector pTXΔ (E), wild-type stp1 (wt), and mutant stp1 strains SRB (G169S) and SRT (Q31X). *, P < 0.01; **, P < 0.009; ***, P < 0.0008; ****, P < 0.0001. Student's t test used to analyze the P values using GraphPad Prism software. Experiments were repeated at least twice.