FIG 4.

In vivo activity of meropenem (MEM) and MEM-ZN148 combination. Neutropenic NMRI mice were inoculated i.p. with ∼5 × 10⁶ CFU of NDM-1-producing K. pneumoniae 50752501 (MEM MIC = 64 mg/liter). Mice were treated by s.c. injection in the neck region with vehicle (PBS), MEM (33 mg/kg), or MEM (33 mg/kg) plus ZN148 (10 mg/kg). Vehicle and ZN148 were administered 1 h postinoculation, whereas MEM was administered 1.5 h postinoculation. Colony counts in blood (A) and peritoneal fluid (B) were determined at 5 h postinoculation. Four mice were included in each group. Groups were analyzed with ANOVA Dunnett´s multiple-comparison test, and P values of <0.05 were considered statistically significant.