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. 2020 May 21;64(6):e00142-20. doi: 10.1128/AAC.00142-20

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FIG 1 — Synteny of optrA loci identified in the Brazilian porcine LR E. faecalis strains. Five different patterns were found (P0, P1, P2, P3, and P4). Areas for recombination identified in the P1 context are marked (red squares). Directions (5′ to 3′) of the primers used to confirm the occurrence of the P0/Tn554/P1-containing circular variants are indicated (red arrows). The primer sets P1F2/P1R2, P1F1/P1R1, and P1F1/P1R2 yielded PCR products corresponding to hp2-ISSep1-like transposase-radC (4,439 bp-P0 circle), SAM-tnpA (6,568 bp-Tn554 circle), and optrA-tnpA (11,007 bp-P1 circle).