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. 2020 May 21;64(6):e00142-20. doi: 10.1128/AAC.00142-20

FIG 3.

FIG 3

optrA alleles SNP-based phylogeny. optrA DNA sequences from the porcine LR E. faecalis isolated in Brazil were compared to all optrA variants available so far in the NCBI database. STs, sources, geographical locations, and GenBank accession numbers are indicated if known (see Table S2 in the supplemental material). A total of 34 OptrA variants are indicated by sequential numbering from top to bottom. The Brazilian OptrA variants (V9, V12, and V13) are marked with asterisks. optrA variants in which SNPs did not yield variations in the 655-amino acid sequence are highlighted by colors. Compared to the OptrA variant first described in China (ST116 E. faecalis E349) (2), variations in the 655-amino acid sequence were detected in V12, V13, and V9 OptrA variants as follows: V12 (Tyr176Asp, Ala350Val, and Gly393Asp), V13 (Tyr176Asp, and Gly393Asp), and V9 (Lys3Glu, Tyr176Asp, Gly393Asp, and Ile622Met).