TABLE 2.
List of strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference, source, or parent strain |
|---|---|---|
| Strains | ||
| E. coli DC10B | Restriction-modification deficient | 59 |
| S. aureus | ||
| AH1263 | USA300 CA-MRSA Erms (LAC) | 60 |
| AH1292 | AH1263 Δagr::tetM | 61 |
| AH1677 | LAC/pDB59 (agr-I) | 40 |
| AH430 | 502A/pDB59 (agr-II) | 40 |
| AH1747 | MW2/pDB59 (agr-III) | 40 |
| AH1872 | MNEV/pDB59 (agr-IV) | 40 |
| AH0845 | USA300 LAC | Parental strain of AH1677 |
| AH0408 | CA-MRSA USA400 (502A) | Parental strain of AH430 |
| AH0843 | CA-MRSA USA400 (MW2) | Parental strain of AH1747 |
| AH0407 | MNEV | Parental strain of AH1872 |
| S. simulans | ||
| AH4549 | MK148 (agr-I) | ATCC 27848 |
| AH5419 | SMU76 (agr-I) | ATCC 31432 |
| AH5420 | KL240 (agr-I) | ATCC 27850 |
| AH5421 | AW215 (agr-I) | ATCC 27849 |
| AH5532 | SNUC1535 (agr-II) | CBMRN |
| AH5533 | SNUC1396 (agr-III) | CBMRN |
| AH5534 | SNUC2478 (agr-III) | CBMRN |
| AH5541 | CNH113 (agr-III) | FDA-ARGOS |
| AH5595 | AH4549/pCM41 | This study |
| AH5596 | AH5532/pCM41 | This study |
| AH5597 | AH5533/pCM41 | This study |
| Coagulase-negative isolates | ||
| AH4551 | Staphylococcus auricularis (WH811M) | ATCC 33753 |
| AH2452 | Staphylococcus capitis | 11 |
| AH3568 | Staphylococcus caprae (DSM 20608) | 11 |
| AH4525 | Staphylococcus cohnii (MF2) | Ward lab |
| AH1740 | Staphylococcus epidermidis | ATCC 12228 |
| AH4513 | Staphylococcus equorum | L. Ackermann |
| AH4547 | Staphylococcus haemolyticus (SM131) | ATCC 29970 |
| AH4545 | Staphylococcus hominis | This study |
| AH2450 | Staphylococcus intermedius | 11 |
| AH2159 | Staphylococcus lugdunensis (N910 320) | 11 |
| AH4522 | Staphylococcus nepalensis (BHW1) | Ward lab |
| AH3604 | Staphylococcus saprophyticus (SST) | ATCC 15035 |
| AH4548 | Staphylococcus warneri (AW25) | ATCC 27836 |
| AH3603 | Staphylococcus xylosus (SXT) | ATCC 29971 |
| Plasmids | ||
| pCM41 | pRNAIII::sGFP | This study |
| pDB59 | P3::YFP | 62 |
a
CA-MRSA, community-acquired methicillin-resistant S. aureus; Erms, erythromycin sensitive.