Skip to main content
. 2020 Jun 2;8(1):e000543. doi: 10.1136/jitc-2020-000543

Figure 3.

Figure 3

PPARγ mediates part of the effects of TFEB in macrophages (MΦs). (A) Luciferase promoter activity assay of the binding of TFEB to PPARγ promoter regions; **p<0.01. (B) Western blot analysis of the indicated protein levels in wild-type (WT) or TFEB transgenic (TFEBtg) bone marrow–derived MΦs (BMDMs) treated with EO771 cell conditioned medium (ECM) with or without GW9662, a PPARγ antagonist. The quantification is shown in online supplementary figure S3. (C) Quantitative real-time PCR analysis of expression of the indicated genes in WT and TFEBtg BMDMs treated with ECM with or without GW9662. n=3; *p<0.05, **p<0.01, ***p<0.001; two-tailed Student’s t-test. Arg1, arginase 1; COX2, cyclo-oxygenase-2; cPLA2, cytosolic phospholipases A2; HIF-1α, hypoxia-inducible factor 1α; IL, interleukin; PPARγ, peroxisome proliferator-activated receptor γ; TFEB, transcription factor EB.