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. 2020 Feb 10;130(3):1168–1184. doi: 10.1172/JCI126361

Figure 5. D1-mGlu5 heteromer is coupled to Gq protein.

Figure 5

(A) CODA-RET enables exclusive monitoring of 1 dimer species of interest. (B–E) CODA-RET imaging monitored on HEK cells expressing the indicated complemented dimers. (B) The pseudocolor images quantify the BRET between the complemented Venus-dimer and Nluc-Gαq protein. (C–E) BRET titration curves were measured for increasing acceptor/donor ratio (Fluo/Lumi). Results were analyzed by nonlinear regression on a pooled data set from 3 independent experiments, assuming a model with 1-site binding (GraphPad Prism 7).