Figure 6. Endogenous D1-mGlu5 clusters are increased in the DA-denervated striatum.

Protein ligation assay (PLA) experiments were performed on intact (A and B) and DA-denervated (C–H, lesioned) striata of WT mice (A–F, WT, n = 7 per treatment condition) and mice with a selective KO of mGluR5 in D1 receptor–expressing neurons (mGluR5^KO-D1; n = 7) (G and H). DA-denervated mice received daily s.c. injection of either L-DOPA (6 mg/kg) (E and F) or vehicle (saline, C and D, G and H) for 3 weeks. All L-DOPA–treated mice developed dyskinesia. (A–H) PLA were obtained with primary antibodies directed against mGlu5 and D1. Plus and minus probes that correspond to secondary antibodies allow rolling-circle amplification and detection of the amplicons by a fluorescence labeled probe (red dots). Nuclei were counterstained with DAPI. Scale bar: 10 μm. (I) Box and whiskers plots of the number of PLA signals (reds dots) per presized zone from WT and KO striatal sections in indicated conditions. Six ROI were analyzed per striatum to measure a mean value per animal. *P < 0.05; ***P < 0.001, Mann-Whitney U test.