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. 2020 Jan 27;130(3):1139–1155. doi: 10.1172/JCI130988

Figure 7. sTDP43 transcripts are enriched in lumbar motor neurons.

Figure 7

(A) Sashimi plots depicting TARDBP splicing in murine frontal cortex homogenate (red) or microdissected lumbar motor neurons (blue). Numbers correspond to exons, and asterisks represent stop codons. (B) Both sTDP43-1 and sTDP43-2 splice events are highly enriched in lumbar motor neurons compared with frontal cortex homogenate. Graph depicts read counts normalized to reads per million for each library. Analysis includes 4 replicates. ****P < 0.0001 by multiple t test with the Holm-Sidak correction. (C) While sTDP43-1 and -2 each comprise approximately 1% of the total TARDBP transcripts in frontal cortex homogenate, they make up 17% and 22% of total TARDBP transcripts in lumbar motor neurons, respectively. Frontal cortex n = 6, lumbar motor neurons n = 4. *P < 0.05, **P < 0.01, ***P < 0.001 by 2-way ANOVA with Sidak’s multiple-comparisons test. (D) sTDP43-1 is enriched within lumbar motor neurons microdissected from both control (n = 9) and sALS (n = 13) patient tissue. (E) sTDP43-1 makes up the majority of total TARDBP transcripts in both control and sALS patient lumbar motor neurons.