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. 2020 Feb 4;130(3):1185–1198. doi: 10.1172/JCI128895

Figure 4. Tumor cells stimulate NK cells to translocate CD73 into the cell membrane and the extracellular space.

Figure 4

(A) Maximum intensity projection of confocal images showing localization of CD73 and CD107a expression in NK cells sorted after tumor coculture under 63× objective lens. Scale bar: 10 μm (n = 4). (B) Overall mean fluorescence intensity (MFI) of CD73 staining per cell comparing CD73+ and CD73– NK cells sorted after tumor coculture (n = 4). (C) MFI of CD73 on cell membrane of every cell comparing CD73+ and CD73– NK cells sorted after tumor coculture (n = 4). Significance was tested by Mann-Whitney U test for both B and C. (D–F) Dose-dependent inhibition of CD73 surface expression on NK cells after 4 hours of coculture with 4-1BBL–transduced K562 in the presence of latrunculin B (D), cytochalasin D (E), or jasplakinolide (F) (n = 4). (G) ELISA showing concentration of CD73 protein released after 24 hours of NK cells cocultured with 4-1BBL–transduced K562 (n = 4). Friedman’s test was used to assess significance in D–F.