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. 2020 Feb 4;130(3):1156–1167. doi: 10.1172/JCI124635

Figure 2. Infiltrating myeloid cell–derived ANXA1 controls muscle repair.

Figure 2

(A) Western blot analysis of ANXA1 protein in total TA muscle. Muscles were analyzed 0, 1, 2, 4, 7, and 14 days after injury. Shown are representative blots (top) and quantification of ANXA1 to β-actin (bottom) and ratios. (B) Quantitative reverse transcriptase PCR analysis of AnxA1 mRNA level in various cell populations FACS-sorted from TA muscle. Muscles were analyzed 0, 1, 2, 4, 7, and 14 days after injury. EC, endothelial cells; FAP, fibro/adipogenic progenitors; SAT, satellite cells; Mac, macrophages; Neut, neutrophils. (C) Experimental setup of bone marrow transplantation (BMT). CX3CR1-GFP mice were irradiated and then transplanted with bone marrow cells isolated from WT or AnxA1–/– mice. Bone marrow engraftment was checked on a blood sample after around 5 weeks. Then animals were injured in their TA by CTX injection and muscles analyzed 0 or 28 days later. Engraftment was confirmed on the bone marrow of each animal on the day of sacrifice. H&E staining (D) and myofiber cross-sectional area (E) of TA muscles 28 days after CTX injury. Scale bar: 50 μm. Results are mean ± SEM of at least 2 (D14 in A) or 3 muscles. *P < 0.05, **P < 0.01 vs. WT or D0.