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. 2020 Feb 10;130(3):1252–1270. doi: 10.1172/JCI131507

Figure 1. Endothelial ZEB1 expression is enhanced in various mouse tumor models.

Figure 1

(A) Immunofluorescent images showing ZEB1 expression in CD31+ blood vessels of transplanted LLC, B16F10, and 4T1 tumors, and lung and kidney tissues (3 mice each). Magnified areas of dashed boxed sections are shown in bottom panels. Arrowheads show ZEB1+CD31+ ECs, and arrows indicate ZEB1+CD31– non-ECs. Nuclei, DAPI (blue). (B) Comparisons of ZEB1 positivity (left) and expression levels (right) in CD31+ blood vessels of transplanted tumors versus normal tissues as shown in A (n = 3 independent experiments). (C) Comparison of Zeb1 mRNA expression in FACS-isolated CD31+CD45– ECs, CD31–CD45+ leukocytes (LCs), and CD31–CD45– other cells (OCs) of LLC tumors (n = 3 independent experiments). Representative flow cytometric plot is shown in left panel. All data are represented as mean ± SD. **P < 0.01. Differences were tested using 1-way ANOVA with Tukey’s post hoc test.