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(A) TUNEL staining of E13.5 embryonic sections uncovered massive apoptosis at the mutant cerebrocortical neuroepithelia. (B) Immunostaining analysis of E13.5 embryonic sections with an anti–activated caspase-3 antibody confirmed massive apoptosis at the mutant cerebrocortical neuroepithelia. Images are representative of 2 experiments. Scale bars: 500 μm (left), 100 μm (middle and right).
