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. 2020 Jun 3;6(23):eaba0745. doi: 10.1126/sciadv.aba0745

Fig. 5. CReP depletion inhibits basal- and amino acid starvation–induced biosynthesis of p27, which is refractory to mTOR inhibition with Torin2.

Fig. 5

(A) HeLa cells with dox-inducible CReP depletion were mock- or dox-treated (~40 hours), treated with Torin2, puromycylated, and lysed at the indicated time points for immunoblot analysis with the indicated antibodies. Statistical significance was determined by two-tailed Student’s t test comparison between the dox/no-dox at the indicated time point (graphs represent mean and SEM, n = 3; **P < 0.005; ***P < 0.0005). (B) Left: Cells with dox-inducible CReP depletion were placed in aa-deprived media (see Materials and Methods) in the presence or absence of Torin2 and lysed at the indicated time points for immunoblot analysis. (B) Right: Cells with dox-inducible CReP depletion were mock- or dox-treated (~40 hours) before being placed in amino acid–deprived media and lysed at the indicated time points for immunoblot analysis. Bar graphs represent mean comparing dox/no dox at indicated time points and SEM. n = 3; *, **, *** corresponds to P < 0.05, 0.005, 0.0005, respectively.