Skip to main content
. 2020 Jun 3;6(23):eaaz1341. doi: 10.1126/sciadv.aaz1341

Fig. 5. Overexpression of barr1 in AgRP neurons improves whole-body glucose tolerance and insulin sensitivity in lean and obese mice.

Fig. 5

Experiments were carried out with male mice maintained on regular chow (RC) (A to I) or with male mice that had consumed an HFD for at least 7 weeks (F to M). (A) Representative immunofluorescence images showing the expression of mCerulean (left), a marker for barr1 overexpression, and mCherry (control mice; right) in the arcuate nucleus of AgRP-barr1-OE and AgRP-mCherry mice, respectively. 3rd v, third ventricle. (B) Glucose tolerance test (1 g glucose/kg i.p.) carried out with regular chow AgRP-barr1-OE and control mice (age, 12 weeks). (C) Insulin tolerance test (0.75 U/kg i.p.) (11-week-old regular chow mice). (D) Fasting and fed blood glucose levels (regular chow mice, 11 to 12 weeks old). (E) Food intake of regular chow AgRP-barr1-OE and control mice after a 24-hour fast (refeeding; mouse age, 13 weeks). (F) Body weight gain of AgRP-barr1-OE and control mice consuming an HFD (mouse age, 13 to 17 weeks). (G) Fat and lean mass composition of HFD AgRP-barr1-OE and control mice (mouse age, 22 weeks; 9 weeks on HFD). (H) Glucose tolerance test (1 g glucose/kg i.p.) carried out with HFD AgRP-barr1-OE and control mice (mouse age, 21 weeks; 8 weeks on HFD). (I) Insulin tolerance test (0.75 U/kg i.p.) (mouse age, 20 weeks; 7 weeks on HFD). (J to M) Fasting and fed blood glucose (J) and plasma insulin (K), FFA (L), and leptin (M) levels (mouse age, 20 to 22 weeks; 7 to 9 weeks on HFD). Data are given as means ± SEM (n = 6 to 9 mice per group). *P < 0.05; **P < 0.01 [two-way ANOVA followed by Bonferroni’s post hoc test (B, C, H, and I) and two-tailed Student’s t test (D, G, and J to M)].