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. 2020 Jun 3;6(23):eaaz1341. doi: 10.1126/sciadv.aaz1341

Fig. 6. Overexpression of barr1 in AgRP neurons protects against diet-induced liver steatosis.

Fig. 6

AgRP-barr1-OE and control mice were maintained on an HFD for at least 9 weeks. (A and B) Liver weights (A) and liver triglyceride levels (B) of HFD AgRP-barr1-OE and control mice. (C) Histological staining of liver sections from HFD AgRP-barr1-OE and control mice. (D) Hepatic expression levels of genes involved in gluconeogenesis and proinflammatory genes in HFD AgRP-barr1-OE and control mice. (E) Liver glycogen levels of HFD AgRP-barr1-OE and control mice. (F) Western blotting analysis of hepatic insulin signaling after intravenous insulin treatment (5 U per mouse) of HFD AgRP-barr1-OE and control mice. (G) Quantification of the Western blotting data shown in (F). Data are given as means ± SEM (n = 4 to 6 male mice per group; mouse age, 24 to 25 weeks, 11 to 12 weeks on HFD). *P < 0.05 (two-tailed Student’s t test).