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. 2020 Jun 3;6(23):eaax8214. doi: 10.1126/sciadv.aax8214

Fig. 4. STK38 is important for ATM activation.

Fig. 4

(A) U2OS cells were transfected with control siRNA and two different STK38 siRNAs. Cells were harvested and lysed with whole-cell lysate buffer or underwent chromatin fractionation or NETN buffer for ATM immunoprecipitation. The samples were blotted with indicated antibodies. (B) U2OS cells were transfected with control siRNA or STK38 siRNA with or without reconstitution of either HA-WT-STK38 or HA-K118R-STK38, and cells were then treated with or without 2 Gy IR. Cell lysates were blotted with indicated antibodies. (C) U2OS cells depleted of STK38 were reconstituted with WT and 4A mutant STK38. Cells were lysed and blotted with indicated antibodies. (D) Analysis of phospho-H3 cells in control siRNA and STK38 siRNA–transfected cells. Means ± SEM are from three experiments. **P < 0.01. Statistical significance was determined by Student’s t test. (E) Analysis of cell cycle distribution of cells transfected with control siRNA or two different STK38 siRNAs. The data presented are means ± SEM for three independent experiments. Statistical significance was calculated using two-way analysis of variance (ANOVA).