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eIF5A promotes a step in translation following initiation. Analysis of [35S]Met incorporation into total peptides (closed symbols) or completed peptides (open symbols) in wild type S. cerevisiae or the eIF5A temperature sensitive mutant (eIF5Ats) at the non-permissive temperature. Ribosome transit times, a metric that is unaffected by translation initiation, are indicated. Red text and lines added to original panels. Panel adapted with permission from Saini et al. 2009.
