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. 2020 Jun 2;4(2):026107. doi: 10.1063/5.0001994

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© 2020 Author(s).

2473-2877/2020/4(2)/026107/11

All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FIG. 3. — Successive confocal microscopy images of the endometrial barrier model. (a) Scheme of the cross sections perpendicular to the Z-axis. (b) The cross sections at indicated depths starting from the epical end of the endometrial layer. Antibodies: for F-actin, Cytopainter Phalloidin-iFluor 488 Reagent (ab176753), 1:1000 in DPBS 1% BSA; for β-tubulin, recombinant anti-beta tubulin antibody conjugated [EPR16774] (Alexa Fluor® 555) (ab206627) diluted with DPBS 1:500; and for nuclei, DAPI (D9542).