Fig. 2.

TLC of E. coli BW25113 pcs lipid extracts confirms PC production. BW25113 pcs was grown in M9 supplemented with 0, 0.01, 0.1, or 1 mM Cho. Rhamnose (0.2%) was used in the medium to induce Pcs expression. Total lipids were extracted and separated with chloroform-methanol-water (65:35:8; v/v/v) on a TLC plate and developed by charring. The data shown are representative of two independent experiments. Samples: 1, POPC (16:0/18:1); 2, E. coli PLE standard; 3, mixture of POPC and PLE standards (1:1); and 4–7, total lipid extracts of E. coli BW25113 pcs grown with 0, 0.01, 0.1, and 1 mM Cho, respectively.