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. 2020 Mar 10;61(6):870–883. doi: 10.1194/jlr.RA120000654

Fig. 4.

Fig. 4.

TLC of E. coli BW25113 and E. coli BW25113 pcs lipid extracts after sucrose gradient separation confirms that PC is present in the IM and OM. Both strains were grown in M9 with 100 µM Cho and 0.2% rhamnose. The cells were harvested. The IM and OM of the lysate were separated via sucrose gradient separation with 2.2 M (bottom) to 0.77 M (top) sucrose concentrations and ultracentrifugation. The fractions were taken in 1 ml steps from the top. Total lipids were extracted from the fractions and separated with chloroform-methanol-water (65:35:8; v/v/v) on a TLC plate and developed by charring. The data shown are representative of two independent experiments. Samples: 1, E. coli K-12 fractions 1–4 (IM); 2, E. coli K-12 fractions 9–12 (OM); 3, E. coli K-12 pcs fractions 1–4 (IM); 4, E. coli K-12 pcs fractions 9–12 (OM); and 5, sucrose standard.