Fig. 7.

Localization of the click-labeled AEPC by plasmolysis. E. coli lptD4213 pcs was grown with 0.5 mM AECho, labeled with Alexa488-DIBO, and stained with FM 4-64 membrane dye. The cells were then plasmolyzed and immediately visualized on a Nikon Eclipse Ti inverted microscope using phase contrast (A) and fluorescence (B, C). The green fluorescent Alexa488 signal (B) localized primarily to the periphery of cells (white arrows). The membranes of E. coli lptD4213 pcs cells stained with FM 4-64 (C) colocalized with the Alexa488 (D; overlay of B and C). The bar represents 3 µm.