Figure 3.

SLC25A18 negatively regulates genes expression related to aerobics glycolysis, cell proliferation, and Wnt/β-catenin cascade. (A) Western blotting verified a negative protein level of genes such as MYC, PKM2, LDHA, AKT1 and pAKT1 which are involved in cell aerobics glycolysis and cell proliferation when SLC25A18 was overexpressed or knocked down. (B) Gene set enrichment analysis implied a negative relationship between highly expressed SLC25A18 and the enrichment of CTNNB1 target genes and Wnt/β-catenin Signaling (HALLMARK_ WNT_BETA_CATENIN_SIGNALING, REACTOME_SIGNALING_BY_WNT) in patients with CRC from the TCGA database, (C) which was also verified by western blotting. All experiments were conducted in triplicates.