Fig. 4.
Effect of VSL#3 supplementation on inflammatory and tight junction proteins in ileums of C57BL/6 (WT) and AppNL-G-F mice. Ileums from the mice were lysed and resolved via SDS-PAGE and western blotted using anti-BACE, anti-GFAP, anti-Iba-1, anti-APP, anti-CD-68, anti-synapsin-1, anti-Cox-2, anti-PSD-95, anti-iNOS, anti-cFos, anti-occludin and anti-GAPDH antibodies. Three representative western blots from 6 animals analyzed are shown in the figure. Quantification shows arbitrary densitometry units of each protein signal normalized to its respective GAPDH loading control. Data are represented as mean ± S.E.M. Significant differences were determined by two-way analysis ANOVA, * p<0.05.