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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Cancer Discov. 2020 Mar 18;10(6):854–871. doi: 10.1158/2159-8290.CD-19-1299

Figure 5: Upregulation of H3K36me2 is essential for EMT.

Figure 5:

(A) Relative mRNA expression of epithelial genes (left) and mesenchymal genes (right) in WT and sgNsd2 clones, with or without 15 days of Tgf-β treatment, as determined by qPCR (n = 3, mean ± SEM).

(B) Western blots of acid-extracted histones from WT and sgNsd2 clones, with or without 15 days of Tgf-β treatment.

(C) Relative mRNA expression of Nsd1, Nsd2, and Nsd3 in WT and sgNsd2 clones, with or without 15 days of Tgf-β treatment, as determined by qPCR (n = 3, mean ± SEM).

(D-E) Western blots of acid-extracted histones (D) and BF images (E) from cell lines expressing WT or K36M H3F3A (scale bars = 100μm). Fold change increases in Ecad mRNA expression in K36M samples were determined by qPCR (see Supplementary Figure S11) and indicated in yellow.

(F-H) Western blots of acid-extracted histones (F), BF images (scale bars = 100μm) (G), and relative mRNA expression of epithelial genes (left) and mesenchymal genes (right) (H) from cells expressing WT or K36M H3F3A, with or without 15 days of Tgf-β treatment. mRNA expression was determined by qPCR (n = 3, mean ±SEM).