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. 2020 May 5;39(23):4538–4550. doi: 10.1038/s41388-020-1307-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a The expression of LINC01278 in HCC cells transfected by miR-1258 and anti-miR-1258. b The TCF-4/LEF-1 binding site in the LINC01278’s promoter sequence was identified. The mutant sequence was designed for the luciferase reporter assay. c The relative luciferase activity was detected in 293 T cells co-transfected by binding-WT/binding-mut and TCF-4. d ChIP assay was used to detect the TCF-4 to the TRE (TCF responsive element: TTCAAAG) regions in LINC01278 promoter. e The relative expression of LINC01278 in HCC cells transfected by β-catenin and siTCF-4. f The relative expression of LINC01278 in HCC cells treated with TGF-β1 (0.5 ng/ml) and siTCF-4. g, h The wound-healing assay and invasion assay in SMMC-7721 cells. i The lung metastasis of SMMC-7721 cells treated with TGF-β1 (0.1 µg/each time, three times a week) and shLINC01278. j Schematic illustration of “β-catenin/TCF-4-LINC01278-miR-1258-Smad2/3” axis. miR-1258, HCC cells were transfected by miR-1258 expression vector. Anti-miR-1258, HCC cells were transfected by miR-1258 antisense plasmid. TCF-4, HCC cells were transfected by TCF-4 expression vector. β-catenin, HCC cells were transfected by β-catenin expression vector. siTCF-4, HCC cells were transfected by siRNA target TCF-4. shLINC01278, HCC cells were transfected by shRNA target LINC01278. *P < 0.05.