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. 2020 May 15;12(5):1942–1953.

Figure 3.

Figure 3

ASPM knockdown blocked the proliferation, migration and invasion of EC cells. A. qRT-PCR was used to measure ASPM expression in HEC-1A and Ishikawa cells after transfection with sh-ASPM or sh-NC. B and C. MTT tests were used to measure the viability of HEC-1A and Ishikawa cells after transfection with sh-ASPM or sh-NC. D. Cell migration was performed in HEC-1A and Ishikawa cells after transfection with sh-ASPM or sh-NC. E. Cell invasion was assessed in HEC-1A and Ishikawa cells after transfection with sh-ASPM or sh-NC. The number of migrated or invaded cells was counted by analysing photographs at 100 × magnification in five random fields per chamber. All samples were stained and evaluated in triplicate. *P<0.05, **P<0.01.