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. 2020 Jun 3;10:9047. doi: 10.1038/s41598-020-65939-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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5′ capture scRNAseq analysis of in vitro DENV infected cells. (A) Schematic representation of the infection, isolation, and analysis strategy utilized in this study. (B) Surface DENV-1 NS1 expression on uninfected, DENV-1 infected, and sorted NS1^pos DENV-1 infected DC-SIGN expressing CEM.NK^R cells utilized for scRNAseq analysis. (C) Number of PE reads confidently mapped from each sample to either the Gh38 human genome assembly or the DENV-1 (strain WestPac74) genome from each sample (D) Fraction of cells from each sample with > =1 confidently mapped DENV-1 transcript present.