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. 2020 Jun 3;10:9047. doi: 10.1038/s41598-020-65939-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characteristics of DENV-1 mapped transcripts identified by 5′ capture scRNAseq. (A) Number of unique DENV-1 mapped transcripts identified per cell in DENV-1 infected DC-SIGN expressing CEM.NK^R cell cultures (blue), DENV-1 infected sorted NS1^pos cultures (red), and DENV-1 infected sorted NS1 ^pos cultures supplemented with a DENV-specific cDNA synthesis primer during scRNAseq analysis (orange). (B) DENV-1 mapped insert lengths identified by scRNAseq analysis. (C) Median DENV-1 genome coverage obtained per UMI in 5′ capture scRNAseq analysis. (D) DENV-1 genome coverage achieved per cell by 5’ capture scRNAseq. Only cells with > =1 DENV-1 mapped UMIs are shown. (E) Relationship between DENV-1 UMI recovery and DENV-1 genome coverage achieved by 5′ capture scRNAseq. Curves calculated by non-linear regression (single variable). **** p < 0.0001, one-way ANOVA.